Fatty acids and plasmalogens of the phospholipids of the sperm membranes and their relation with the post-thaw quality of stallion spermatozoa.
نویسندگان
چکیده
Fatty acids and plasmalogens were extracted from the phospholipids of the plasma membrane of stallion spermatozoa, to determine their relation with sperm quality after freezing and thawing. Sperm quality was rated using a quality index that combined the results of the analysis of sperm motility and velocity (CASA analysis), membrane status and mitochondrial membrane potential (flow cytometry) post thaw. Receiving operating system (ROC) curves were used to evaluate the value of specific lipid components of the sperm membrane herein studied as forecast of potential freezeability. From all parameters studied the ratio of percentage of C16 plasmalogens related to total phospholipids was the one with the better diagnostic value. For potentially bad freezers, the significant area under the ROC-curve was 0.74, with 75% sensitivity and 79.9% specificity for a cut off value of 26.9. Also the percentage of plasmalogens respect to total phospholipids gave good diagnostic value for bad freezers. On the other hand, the percentage of C18 fatty aldehydes related to total phospholipids of the sperm membrane properly forecasted freezeability with an area under the ROC curve of 0.70 with 70% sensitivity and 62.5% specificity for a cut off value of 0.32.
منابع مشابه
I-12: Omega-3 Fatty Acids Accompanied with ?-Tocopherol Improved Fresh and Post-Thaw Sperm Quality in Ruminants
متن کامل
O-16: Metabolism of Exogenous Fatty Acids, Fatty Acid-Mediated Cholesterol Efflux, PKA and PKC Pathways in Boar Sperm Acrosome Reaction
Background: For understanding the roles of fatty acids on the induction of acrosome reaction which occurs under association of cholesterol efflux and PKA or PKC pathways in boar spermatozoa, metabolic fate of alone and combined radiolabeled 14C-oleic acid and 3H-linoleic acid incorporated in the sperm was compared, and behavior of cholesterol and effects of PKA and PKC inhibitors upon fatty aci...
متن کاملP-113: Quality Improvement of Buffalo Frozen -Thawed Spermatozoa by Supplementation of Cysteine and Glutamine in Cryopreservation Extender
Background: Sperm cryopreservation has been associated with over production of reactive oxygen species (ROS). Buffalo spermatozoa are susceptible to ROS inducing damages due to insufficient level of cytoplsmic antioxidant along with high amount of poly unsaturated fatty acids composition in membrane structure. Therefore, appropriate antioxidant in buffalo cryopreservation extender would reduce ...
متن کاملP-55: Trolox (Vitamin E) Improves SpermQuality in Oligospermia during Cryopreservation
Background: Since the deleterious effects of reactive oxygen species (ROS) during sperm cryopreservation can be overcome by antioxidants, post-thaw human sperm quality may perhaps profit from adding antioxidants to the extender media prior to cryopreservation. This study evaluated the effects of different concentrations of Trolox supplementation to cryo-protective media on post-thaw motility an...
متن کاملBull Fertility and Its Relation with Density Gradient Selected Sperm
Background sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. Few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (PT) or after selecting sperm sample (SSp), using density gradient separatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Theriogenology
دوره 75 5 شماره
صفحات -
تاریخ انتشار 2011